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SARS-CoV-2 (Delta Variant) Virucidal Efficancy Tests on 100 Times Washed Copper Line Mask
JOHN HWA Lee, DVM, PhD College of Veterinary Medicine, Jeonbuk National University, South Korea
Test Results: Copper Line Mask inactivates COVID-19 Delta Variant over 80% within 10 minutes and over 98% in 30 minutes and almost the same results for both original and 100 washed samples.
The tests were conducted from July to August, 2021. SARS-CoV-2 (hCoV-19/Korea/KDCA119861/2021) was provided by the Korea Centers for Disease Control and Prevention (KCDC) and was propagated in Vero E6 cells. All experiments conducted and using SARS-CoV-2 were performed at Jeonbuk National University Zoonotic Center, using enhanced biosafety level 3 (BSL3) containment procedures in laboratories approved for use by the KCDC.
Testing Mask Samples:
- Non-Washed Copper Line Mask (0 Time Washes)
- 100 Times Washed Copper Line Mask (The mask was washed 100 times with neutral detergent contained water (luke warm at 30°C +/-3°C) for 5-7 minutes each time and was dried before using.
Methodology:
- Cytopathic Effects (CPE)
- Immunofluorescence Assay (IFA)
- Quantitative Real-Time PCR (q-PCR)
- Plaque Reduction Assay – Determination by plaque reduction assay (followed according to the guide line of ISO18184)
IFA (Immunofluorescence Assay
q-PCR (Quantitative Real-Time PCR)
PRA (Plaque Reduction Assay) — Determination by plaque reduction assay (followed according to the guide line of ISO18184)
Revolutionary Copper Line Face Mask Scientifically Proven to Deactivate COVID-19 (SARS-CoV2) and its Strongest Variant, Delta Variant
Asia’s Largest and Leading Microbiology Research Institute Confirms Copper Line’s Unrivalled Efficacy and Protection
Copper Line Mask is the first and only face mask with the proven ability to render the COVID-19 virus inactivate and terminate the replication of its RNA, according to the tests conducted by the BSL3 laboratory from July 7th to Aug 19th, 2020 at Jeonbuk National University, South Korea, the largest microbiology research institute in Asia. This accredited laboratory tested the neutralization capacity and reusability of masks infused with copper against regular masks without copper when exposed to the COVID-19 virus (SARS-CoV2).
And according to the latest tests on July to August 2021, even after 100 times washes, Copperline Masks can deactivate COVID-19 and its the strongest variant, Delta Variant over 80% in 10 mins. and almost completely within 30 mins.. In addition, our mask can kill all kinds of enveloped viruses, which includes all respiratory viruses including flu.
In an immunofluorescence assay, the fluorescence signal was almost undetected in cells treated with Copper Line Mask’s 22%-copper-infused material for 30 minutes, in contrast with an intense green fluorescence signal in the group treated with 0% copper. This finding was reinforced by a quantitative real-time PCR test, which observed a pronounced decline in the relative expression of the SARS-CoV2.
In terms of reusability, the mask was found to retain its virus-deactivation capacity even after 30 washes.
Dr. John Hwa LEE, DVM, PhD, a professor and Dean at the Jeonbuk National University who led the studies, said, “The tests conclude that copper can quickly and effectively render SARS-CoV2 inactivate, inhibit the replication of its RNA and reduce the infectivity of the virus. This is due to copper’s oxidation behavior and DNA affinity. Copper can bind with and disorder helical structures, and breach the integrity of the virus plasma membrane, thus killing the virus. In short, copper is essential and powerful tool in slowing the spread of COVID-19.”
“Additionally, Copper Line Mask’s material which is 3D textured with 22% copper further enhances virucidal activity against SARS-Cov2. The material’s 3D structure has a higher capacity to kill the virus remain in the mask. Increased surface area improves microbicidal action through the interaction of copper ions with virions entrapped within the mask or in contact with the mask’s copper-infused surface.”
In terms of reusability, the mask was found to retain its virus-deactivation capacity even after 30 washes.
Dr. John Hwa LEE, DVM, PhD, a professor and Dean at the Jeonbuk National University who led the studies, said, “The tests conclude that copper can quickly and effectively render SARS-CoV2 inactivate, inhibit the replication of its RNA and reduce the infectivity of the virus. This is due to copper’s oxidation behavior and DNA affinity. Copper can bind with and disorder helical structures, and breach the integrity of the virus plasma membrane, thus killing the virus. In short, copper is essential and powerful tool in slowing the spread of COVID-19.”
“Additionally, Copper Line Mask’s material which is 3D textured with 22% copper further enhances virucidal activity against SARS-Cov2. The material’s 3D structure has a higher capacity to kill the virus remain in the mask. Increased surface area improves microbicidal action through the interaction of copper ions with virions entrapped within the mask or in contact with the mask’s copper-infused surface.”
While copper’s antiviral and antibacterial properties have long been known, the superiority of copper ions over other metal ions has also been proven by Dr. LEE. In a related test, copper ions were tested alongside seven other metals, including Iron, Aluminum and Chromium. Copper ions were observed to have about five times the ability to disrupt RNA structures compared to that of the seven other metal ions.